Thermo Fisher Scientific ADFP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000
• View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100
• View publication

Product Specifications

Product Specific Information

• Recommended positive controls: H1299, HepG2
• Predicted reactivity: Mouse (82%), Pig (87%), Sheep (86%), Chimpanzee (97%), Bovine (86%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Milk lipid globules from humans, cows, and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.

ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression of adipophilin is restricted to specific cell types such as:

• Lactating mammary epithelial cells
• Adrenal cortex cells
• Sertoli and Leydig cells of the male reproductive system
• Hepatocytes undergoing steatosis in alcoholic liver cirrhosis

ADFP may serve as a marker for identifying specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.