Thermo Fisher Scientific PLA2G4A Polyclonal Antibody
PLA2G4A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 적합. Human 및 Mouse 반응성 확인됨. 항원 친화 크로마토그래피로 정제되었으며, 장기 보관 시 -20°C 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–263 of Human PLA2G4A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546576 |
Product Specific Information
- Recommended positive controls: Raji, NIH-3T3
- Predicted reactivity: Mouse (95%), Rat (95%), Rabbit (92%), Bovine (92%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid, which is subsequently metabolized into eicosanoids.
Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways.
The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor.
The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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