Thermo Fisher Scientific APOBEC3C Polyclonal Antibody

Thermo Fisher Scientific APOBEC3C Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

APOBEC (apolipoprotein B mRNA editing enzyme, catalytic) proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA.
The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22.
Like APOBEC3G, APOBEC3F deaminates deoxycytosine to deoxyuracil in the minus strand of HIV-1 DNA, resulting in G to A hypermutation in the plus strand of DNA.
Thus, APOBEC3G and APOBEC3F provide a mechanism for innate immunity to retroviruses and contribute to sequence variation observed in many viruses.
Viral infectivity factor (Vif) imparts APOBEC3G and APOBEC3F resistance to HIV through impaired translation of their mRNA and accelerated posttranslational degradation of APOBEC3 proteins by the 26S proteasome.
Interestingly, HIV-1 Vif cannot form a complex with APOBEC3G or APOBEC3F of mouse origin as it does with the human protein, and thus mouse APOBEC3G and APOBEC3F function as potent inhibitors of wild-type HIV-1 replication, whereas human APOBEC3G and APOBEC3F only inhibit Vif-deficient HIV-1 replication.
This implies that induction of APOBEC3G and APOBEC3F activity or blocking their interaction with Vif may provide a therapeutic intervention method.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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