Thermo Fisher Scientific Phospho-PLC gamma 1 (Tyr771) Polyclonal Antibody
Phospho-PLC gamma 1 (Tyr771) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human PLC gamma 1 around Tyr771 phosphorylation site |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Phospholipase C gamma (PLCγ) family in mammals includes PLCγ1 and PLCγ2, which share core structural domains and unique regions containing an additional PH domain, two SH2 domains, and one SH3 domain. Upon extracellular stimulation by hormones or growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. The activated enzyme catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), generating second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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