Thermo Fisher Scientific PHAP Polyclonal Antibody
PHAP 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 세포 사멸 관련 연구에 적합하며, Raji cell lysate를 양성 대조로 권장. 액상 형태로 1 mg/mL 농도, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide (Human) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_1959538 |
Product Specific Information
- Recommended positive controls: Raji whole cell lysate
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Apoptosis is related to many diseases and developmental processes. Caspase-9 plays a central role in cell death induced by various apoptosis activators. After cytochrome c is released from mitochondria, it binds to Apaf-1 to form an apoptosome that activates procaspase-9. Activated caspase-9 subsequently cleaves and activates effector caspases (caspase-3, -6, and -7), which are responsible for the proteolytic cleavage of key proteins in apoptosis.
The tumor suppressor putative HLA-DR-associated proteins (PHAPs) have been identified as important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation and stimulates the mitochondrial apoptotic pathway. It also opposes both Ras- and Myc-mediated cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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