Thermo Fisher Scientific SPAG1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SPAG1. Recombinant protein control fragment (Product #RP-103163). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPAG1,uniProtId:Q07617-1,ncbiNodeId:9606,antigenRange:152-233,antigenLength:926,antigenImageFileName:PA5-62825_SPAG1_Q07617-1_Rabbit.svg,antigenImageFileNamePDP:PA5-62825_SPAG1_Q07617-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647773

Product Specific Information

Immunogen sequence: KKTPRDYAEW DKFDVEKECL KIDEDYKEKT VIDKSHLSKI ETRIDTAGLT EKEKDFLATR EKEKGNEAFN SGDYEEAVMY YT

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 87%.

Target Information

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.