Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Rat IgG에 특이적인 염소 유래 교차흡착 2차 항체로 DyLight 550 형광 표지. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 비특이적 반응 최소화 및 높은 신호 대비 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556599 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, human, mouse, rabbit, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, human, mouse, rabbit, and sheep, ensuring high specificity in immunoassays.
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies increase assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. They are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for enhanced specificity.
Additional Formats
Available in multiple conjugates, including:
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5
- DSB-X Biotin, DyLight 488, 594, 650, 680, 755, 800
- FITC, HRP, PE, Texas Red-X, TRITC
- Custom conjugation upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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