
Thermo Fisher Scientific Goat anti-Rat IgM Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광 표지를 가진 염소 유래 항-Rat IgM 2차 항체로, 인간 및 마우스에 대한 교차 반응을 최소화함. Western blot, IHC, ICC, Flow cytometry 등 다양한 면역 분석에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체.
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Thermo Fisher Scientific Goat anti-Rat IgM Cross-Adsorbed Secondary Antibody, DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgM |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556593 |
| Target | IgM |
| Cross Adsorption | Against human and mouse |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to human and mouse.
Target Information
Anti-Rat secondary antibodies are affinity-purified and exhibit high specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat targets. Secondary antibodies enhance versatility and sensitivity in various detection systems (HRP, AP, fluorescence) through signal amplification. Typically, secondary antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 680
- DyLight 755
- DyLight 800
- Request custom conjugation
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