Thermo Fisher Scientific Insulin Receptor Alpha Recombinant Rabbit Monoclonal Antibody (INSR, 2277R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of phosphorylation cascades.
Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins.
Defects in insulin receptor (IR) are associated with insulin resistance syndromes, while IGF-1R defects may cause growth retardation.
Both signaling cascades are important in cancer development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.