Thermo Fisher Scientific HLA-DOA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000
• Publications: 참고문헌 보기

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500
• Publications: 참고문헌 보기

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total HLA-DOA.

Target Information

Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively.
DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is selectively expressed on antigen-presenting cells such as B cells, dendritic cells, and thymic epithelial cells.
DO enhances peptide loading efficiency and stabilizes DM at low pH, preserving its chaperon activity. DO–DM complexes are more efficient than DM alone in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: PA5-101633_HLA-DOA_P06340-1_Rabbit.svg, PA5-101633_HLA-DOA_P06340-1_Rabbit_PDP.jpeg)