Thermo Fisher Scientific AGO3 (Argonaute-3)/eIF2C3 Monoclonal Antibody (PCRP-AGO3-1C5)
AGO3 단백질을 인식하는 마우스 단일클론 항체로, RNA 매개 유전자 침묵 연구에 적합함. ICC/IF, Flow Cytometry, ELISA, IP 등 다양한 응용 가능. Human 반응성, Protein A/G 정제, 4°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific AGO3 (Argonaute-3)/eIF2C3 Monoclonal Antibody (PCRP-AGO3-1C5)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1–2 µg/100–500 µg total protein |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-AGO3-1C5 |
| Immunogen | Recombinant full-length human AGO3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
AGO3 (protein argonaute-3) is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) and represses the translation of complementary mRNAs. AGO3 is proposed to stabilize small RNA derivatives (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells. It may also mediate riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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