Thermo Fisher Scientific SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

1:5,000-1:10,000

-

Neutralization (Neu)

-

View 1 publication 1 publication

Immunoelectrophoresis (IE)

-

- -

Product Specifications

Published species

Human, Plant, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human SARS Coronavirus Spike S1 Subunit Protein (Met1-Arg667)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Wet ice

RRID

AB_2788969

Product Specific Information

PA5-81795 has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 and SARS-CoV-2 (2019-nCoV) Spike RBD.

PA5-81795 was prepared using the sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667). This was expressed with a C-terminal polyhistidine tag.

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human SARS Coronavirus Spike S1 Subunit Protein.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.