Thermo Fisher Scientific SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody
SARS/SARS-CoV-2 스파이크 단백질(S1) 특이 토끼 폴리클로날 항체로, WB, ELISA 등 다양한 응용에 적합. SARS-CoV 및 SARS-CoV-2 RBD와 교차 반응. 보존제 무첨가, 장기 보관 시 -20°C 권장. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 5 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| ELISA | 1:5,000–1:10,000 | View publications |
| Neutralization (Neu) | – | View 1 publication |
| Immunoelectrophoresis (IE) | – | View publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human, Plant, Virus |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human SARS Coronavirus Spike S1 Subunit Protein (Met1–Arg667) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 1 month. For long-term storage, store at –20°C. |
| Shipping conditions | Wet ice |
| RRID | AB_2788969 |
Product Specific Information
- PA5-81795 shows cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 and SARS-CoV-2 Spike RBD.
- Prepared using the sequence encoding the S1 subunit of SARS-CoV (isolate: WH20) spike (AAX16192.1) (Met1–Arg667), expressed with a C-terminal polyhistidine tag.
- Preservative-free; sodium azide (0.05–0.1%) recommended to prevent contamination.
- Specific for Human SARS Coronavirus Spike S1 Subunit Protein.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses from mild cold-like symptoms to severe respiratory distress and death. The SARS-CoV-2 Spike Protein consists of S1 and S2 domains and serves as the main surface antigen of the virus.
The glycosylated Spike protein, along with the nucleocapsid protein, can be detected in infected cell culture supernatants using antisera from SARS patients.
S1 contains a receptor-binding domain (RBD) that specifically binds to angiotensin-converting enzyme 2 (ACE2) on target cells.
Variations in Spike proteins reflect CoV diversity, affecting receptor interactions and viral membrane fusion mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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