Thermo Fisher Scientific Phospho-MCM4 (Ser88) Polyclonal Antibody
Phospho-MCM4 (Ser88) 폴리클로날 항체로 인간, 마우스, 비인간 영장류, 랫트 반응성. WB, IHC, ICC/IF에 사용 가능. 인산화된 Ser88 위치에서 MCM4 단백질을 특이적으로 검출. 고순도 정제 및 안정적 PBS/glycerol buffer 형태로 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MCM4 (Accession P33991), corresponding to amino acid residues around phosphorylated Ser88 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816990 |
Product Specific Information
Antibody detects endogenous levels of MCM4 only when phosphorylated at Ser88.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric MCM complex is a key component of the pre-replication complex (pre_RC) and may be involved in replication fork formation and recruitment of other DNA replication-related proteins.
The MCM complex consisting of MCM2, MCM4, MCM6, and MCM7 possesses DNA helicase activity and may act as a DNA unwinding enzyme. Phosphorylation of this protein by CDC2 kinase reduces DNA helicase activity and chromatin binding of the MCM complex.
This gene is located on chromosome 8, adjacent to PRKDC/DNA-PK, a DNA-activated protein kinase involved in double-strand break repair. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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