Thermo Fisher Scientific TACC3 Monoclonal Antibody (OTI1E11), TrueMAB
TACC3 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1E11). Western blot, ICC/IF, Flow cytometry에 사용 가능. Human 및 Rat 반응성. Lyophilized 형태, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E11 |
| Immunogen | Full length human recombinant protein of human TACC3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 100 µL distilled water to obtain a final antibody concentration of ~1 mg/mL.
- For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TACC1 is located on the 8p11 chromosomal region, amplified in approximately 15% of breast tumor samples. The short arm of chromosome 8 also contains FGFR1, which is overexpressed in most breast cancers.
TACC family members (TACC1, TACC2, TACC3) are closely mapped to FGFR1, FGFR2, and FGFR3 genes on chromosomes 4, 8, and 10, respectively.
Because of their phylogenetic relationship, TACC and FGFR are proposed to have similar roles in cell growth and differentiation.
TACC1 contains a conserved C-terminal region similar to the Drosophila homolog D-TACC, which is essential for normal spindle function. Mammalian TACC proteins interact with centrosomes and microtubules in a similar manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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