Thermo Fisher Scientific Calprotectin/MRP14/S100A9/Calgranulin B Monoclonal Antibody (MRP14/840)
인간 Calprotectin/MRP14/S100A9 단백질을 인식하는 mouse monoclonal antibody. 다양한 면역염색 및 유세포분석에 활용 가능. 단백질 A/G로 정제된 액상형, 보존제 무첨가. 연구용으로만 사용.
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Thermo Fisher Scientific Calprotectin/MRP14/S100A9/Calgranulin B Monoclonal Antibody (MRP14/840)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MRP14/840 |
| Immunogen | Recombinant human MRP14 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The S100A8/A9 heterocomplex, also known as MRP-8/MRP-14 complex or calprotectin, constitutes approximately 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen both extra- and intracellularly, while neutrophils carry it only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulating, and antiproliferative effects, and serves as a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, such as inflammatory bowel disease.
Granulocytes terminate their existence after transmigration through the intestinal wall, making calprotectin detectable in feces. Elevated levels of calprotectin have been observed in plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infections and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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