Thermo Fisher Scientific CD31/PECAM-1 Monoclonal Antibody (PECAM1, 3525)
CD31/PECAM-1 단클론 항체로 인간 시료에 반응합니다. Western blot, IHC(P), Peptide Array에 사용 가능합니다. 단백질 A/G로 정제된 액상 형태이며, PBS(pH 7.4) 버퍼에 보존제가 없습니다. 혈관 내피 및 면역세포 연구에 적합합니다.
- 카탈로그번호
- 5175-MSM15-P1ABX
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PECAM1, 3525 |
| Immunogen | Recombinant fragment (around aa 625–738) of human CD31 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling via dual immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment, mainly at junctions between adjacent cells.
The N-terminal Ig-like domain of CD31 mediates homophilic binding, crucial for cell-cell interactions. CD31 plays diverse roles in:
- Modulation of integrin-mediated cell adhesion
- Transendothelial migration and angiogenesis
- Apoptosis regulation
- Negative regulation of immunoreceptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
It is one of the key regulatory molecules in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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