Thermo Fisher Scientific eIF4B Polyclonal Antibody
eIF4B 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, ICC/IF, ELISA, IP에 적합. Human 및 Mouse 시료에 반응하며, Affinity Chromatography로 정제됨. PBS/glycerol buffer에 보관되며 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 352–611 of human EIF4B (NP_0014082) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.27 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806507 |
Product Specific Information
Immunogen sequence:
SQSTRAASIF GGAKPVDTAA REREVEERLQ KEQEKLQRQL DEPKLERRPR ERHPSWRSEE TQERERSRTG SESSQTGTST TSSRNARRRE SEKSLENETL NKEEDCHSPT SKPPKPDQPL KVMPAPPPKE NAWVKRSSNP PARSQSSDTE QQSPTSGGGK VAPAQPSEEG PGRKDENKVD GMNAPKGQTG NSSRGPGDGG NRDHWKESDR KDGKKDQDSR SAPEPKKPEE NPASKFSSAS KYAALSVDGE DENEGEDYAE
Positive Samples: 22Rv1, HeLa, 293T, M21
Target Information
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5′-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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