Thermo Fisher Scientific eIF4B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_0014082) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF4B,uniProtId:P23588-1,ncbiNodeId:9606,antigenRange:352-611,antigenLength:611,antigenImageFileName:PA5-92449_eIF4B_P23588-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92449_eIF4B_P23588-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806507

Product Specific Information

Immunogen sequence: SQSTRAASIF GGAKPVDTAA REREVEERLQ KEQEKLQRQL DEPKLERRPR ERHPSWRSEE TQERERSRTG SESSQTGTST TSSRNARRRE SEKSLENETL NKEEDCHSPT SKPPKPDQPL KVMPAPPPKE NAWVKRSSNP PARSQSSDTE QQSPTSGGGK VAPAQPSEEG PGRKDENKVD GMNAPKGQTG NSSRGPGDGG NRDHWKESDR KDGKKDQDSR SAPEPKKPEE NPASKFSSAS KYAALSVDGE DENEGEDYAE; Positive Samples: 22Rv1, HeLa, 293T, M21

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5`-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.