
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
HRP 결합된 Goat anti-Rabbit IgG (H+L) 2차 항체로 Western blot, IHC, ELISA 등에 사용 가능. 높은 특이성과 감도를 제공하며, 친화 크로마토그래피로 정제됨. 다양한 형광 및 효소 결합 형식으로도 제공됨. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2534770 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Each lot’s sensitivity is confirmed using ELISA.
- Specificity is verified by immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL deionized water and let stand 30 minutes at room temperature to dissolve.
(Product has been overfilled to ensure complete recovery.) - Centrifuge to remove particulates and prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins.
They are useful in detection, sorting, or purification of rabbit IgG targets.
Secondary antibodies offer versatility for various detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity via signal amplification.
Typically generated by immunizing a host animal with pooled immunoglobulins from the target species, they can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- Biotin
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available upon request
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