Thermo Fisher Scientific Phospho-gamma-H2AX (Ser139) Polyclonal Antibody
DNA 손상 및 복구 연구용 항체로, 인산화된 gamma-H2AX(Ser139)을 특이적으로 인식합니다. IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 인간 및 마우스 반응성이 확인되었습니다. 항원 친화 크로마토그래피로 정제된 고품질 rabbit polyclonal 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | 1:100–1:500 | — |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 | 2 publications available |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region surrounding phosphorylated serine 139 of human histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig, Pig, Monkey, Gorilla, Little brown bat, Thirteen-lined ground squirrel, and Chinese hamster.
Target Information
H2AX is a variant of the H2A protein family and a component of the histone octamer in nucleosomes. It becomes phosphorylated at Ser139 (γ-H2AX) by kinases such as ATM and ATR in the PI3K pathway. γ-H2AX formation marks the initiation of DNA double-strand break (DSB) repair by recruiting DNA repair proteins. The presence of γ-H2AX foci serves as a sensitive biomarker for DNA damage induced by ionizing radiation or cytotoxic agents. Visualization of γ-H2AX enables assessment of genomic damage and DNA repair mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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