Merck Anti-α Actinin antibody, Mouse monoclonal
비근육성 α-액티닌-1을 인식하는 mouse monoclonal 항체로, F-actin 교차결합 단백질 연구에 적합. Western blot 및 immunofluorescence에 사용 가능. 인간 시료 반응성, IgM 동형, −20°C 보관.
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Anti-α Actinin Antibody, Mouse Monoclonal
Clone Information
- Clone: BM-75.2
- Source: Purified from hybridoma cell culture
- Isotype: IgM
Target Description
Alpha-actinin is an actin-binding protein found in both muscle and non-muscle cells. It has a molecular weight of approximately 100 kDa and forms dimers in solution.
In skeletal muscle, α-actinin is localized to the Z-discs defining sarcomeres, while in smooth muscle it is found in dense bodies and plaques.
This antibody recognizes the non-muscle α-actinin-1 isoform, an F-actin cross-linking cytoskeletal protein.
Product Specifications
| 항목 | 내용 |
|---|---|
| Biological Source | Mouse |
| Antibody Type | Primary antibody |
| Clone | BM-75.2, monoclonal |
| Molecular Weight | ~100 kDa |
| Species Reactivity | Human |
| Packaging | 25 μL small pack |
| Concentration | ~1 mg/mL |
| Techniques | Immunoblotting: 0.5–1 μg/mL (HeLa extract) Immunofluorescence: 5–10 μg/mL (Hs68 fibroblasts) |
| Isotype | IgM |
| UniProt Accession | P12814 |
| Shipping Condition | Dry ice |
| Storage Temperature | −20 °C |
| Gene Information | human ACTN1(87) |
Additional Information
Monoclonal Anti-α Actinin (mouse IgM) is derived from hybridoma BM-72.5, produced by fusion of mouse myeloma cells with splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.
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