Merck Anti-LC3C antibody, Mouse monoclonal

Anti-LC3C antibody, Mouse monoclonal

clone LC3C-37, purified from hybridoma cell culture, affinity isolated antibody

항체 형태

affinity isolated antibody
purified from hybridoma cell culture

antibody product type

primary antibodies

클론

LC3C-37, monoclonal

species reactivity

human

포장

antibody small pack of 25 μL

농도

~1 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LC3C.
immunofluorescence: 2.5-5 μg/mL using human HeLa cells treated with Bafilomycin A1 for autophagosomes accumulation

동형

IgG1

UniProt 수납 번호

Q9BXW4

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... MAP1LC3C(440738)

At least 16 genes encoding for autophagy (ATG) related proteins that are required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals.6 The Microtubule-associated protein light chain 3 (MAP1LC3), the mammalian homolog of yeast Atg8/Apg8/Aut7, is essential for the formation of autophagosomes.7 In addition, three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues.8The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa). LC3-II is the result of LC3-I cleavage at the C-terminal Glycine by cysteine protease Atg4, which is further modified by the mammalian E1- and E2-like enzymes: Atg7 and Atg3.7 The levels of LC3-II protein correlates with the extent of autophagosome formation.3 Thus, LC3C can be used as an autophagosomal and mitophagy marker.3,7,9-11