Merck Monoclonal Anti-polyHistidine antibody produced in mouse

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, purified immunoglobulin

Monoclonal His6 tag, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Hexa His tag, Monoclonal Histidine tagged, Monoclonal His-tag, Monoclonal HHHHHH epitope tag, Monoclonal Poly-His-tag

Quality Level

200

생물학적 소스

mouse

항체 형태

purified immunoglobulin

antibody product type

primary antibodies

클론

HIS-1, monoclonal

포장

antibody small pack of 25 μL

농도

~1.0 mg/mL

technique(s)

flow cytometry: 3-6 μg/test
immunoblotting: 0.25-0.5 μg/mL
immunofluorescence: 4-8 μg/mL

동형

IgG2a

저장 온도

−20°C

Monoclonal Anti-polyHistidine (mouse IgG2a isotype) is derived from the HIS-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant His-tagged fusion protein. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal anti-polyHistidine recognizes native as well as denatured, reduced forms of synthetic polyHistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET, and pTrc expression vectors. The antibody preferentially recognizes N-terminal tagged fusion proteins.