Merck Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse

clone GFP-20, ascites fluid

GFP Antibody - Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, Gfp Antibody Sigma, Gfp Monoclonal Antibody

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

antibody product type

primary antibodies

클론

GFP-20, monoclonal

분자량

antigen 27 kDa

포함

15 mM sodium azide

포장

antibody small pack of 25 μL

technique(s)

dot blot: suitable
indirect ELISA: suitable
western blot: 1:2,000 using purified recombinant GFP preparation

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.