Merck Trypsin from bovine pancreas

Trypsin from bovine pancreas

Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein

Quality Level

200

생물학적 소스

bovine pancreas

유형

Type XI

form

lyophilized powder

specific activity

≥6,000 BAEE units/mg protein

분자량

23.8 kDa

구성

protein, 90-100%

불순물

salt, essentially free

solubility

hydrochloric acid: soluble 1 mM, clear

외래 활성

Chymotrypsin ≤0.1 BTEE units/mg protein

저장 온도

−20°C

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.