Merck Carboxypeptidase B from porcine pancreas

Carboxypeptidase B from porcine pancreas

lyophilized powder

Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase

생물학적 소스

Porcine pancreas

Quality Level

200

form

lyophilized powder

specific activity

≥125 units/mg protein

분자량

34,000 Da± 600

정제법

affinity chromatography

구성

Protein, 40-70%

외래 활성

carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%

저장 온도

−20°C

InChI

1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChI key

TWURVFFNODFJBJ-UHFFFAOYSA-N

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.