Merck 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC)

Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), AzBTS-(NH4)2

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

water: 10 mg/mL, clear to slightly hazy

저장 온도

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:

• ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
• ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.