Merck Monoclonal Anti-α-Tubulin antibody produced in mouse

Monoclonal Anti-α-Tubulin antibody produced in mouse

clone DM1A, ascites fluid

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

antibody product type

primary antibodies

클론

DM1A, monoclonal

분자량

antigen ~50 kDa

포함

15 mM sodium azide

species reactivity

bovine, rat, yeast, human, mouse, chicken, fungi, amphibian

향상된 검증

independent ( antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts

동형

IgG1

UniProt 수납 번호

P68366

application(s)

research pathology

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.