Merck Klenow Enzyme

Klenow Enzyme

Sequencing grade, from Escherichia coli Iysogenic for NM 964

Klenow

생물학적 소스

Escherichia coli

Quality Level

100

form

solution

specific activity

>5000 units/mg protein

분자량

Mr 75 kDa

포장

pkg of 250 U (5 x 103 U/ml)

manufacturer/tradename

Roche

배송 상태

dry ice

저장 온도

−20°C (−15°C to −25°C)

Klenow enzyme is the large, C-terminal fragment (Mr 76,000) of E.coli DNA polymerase I, which can be obtained by subtilisin treatment of intact DNA polymerase I. It retains the 5′→3′ polymerase and the 3′→5′ exonuclease activities of intact DNA polymerase I, but lacks the 5′→3′ exonuclease activity of the native enzyme. The enzyme catalyzes the addition of mononucleotides from deoxynucleoside-5′-triphosphates to the 3′-hydroxyl terminus of a primer/template DNA. This property is used to synthesize DNA complementary to single-stranded DNA templates.
Klenow Enzyme is formed by subtilisin treatment of DNA polymerase I.