Merck Pwo SuperYield DNA Polymerase, dNTPack

Pwo SuperYield DNA Polymerase, dNTPack

sufficient for ≤40 reactions, pkg of 100 U, optimum reaction temp. 72 °C

PCR | Polymerase

사용

sufficient for ≤40 reactions

Quality Level

100

포장

pkg of 100 U

manufacturer/tradename

Roche

파라미터

72 °C optimum reaction temp.

배송 상태

dry ice

저장 온도

−20°C (−15°C to −25°C)

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction without changing the fidelity of DNA synthesis. This enzyme delivers excellent results due to its enzyme design and optimized buffer system. It amplifies fragments up to 3 kb - even longer amplicons are possible from simple templates.
Pwo DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.

Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5′ → 3′ DNA polymerase with 3′ → 5′ exonuclease proofreading activity; 5′ → 3′ exonuclease activity is not detectable.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5 mM.