Merck DNA Molecular Weight Marker II

DNA Molecular Weight Marker II

form

solution

Quality Level

100

포장

pkg of 50 μg (in 200 μl)

manufacturer/tradename

Roche

저장 조건

avoid repeated freeze/thaw cycles

배송 상태

dry ice

DNA Molecular Weight Marker II is a ready-to-use solution, 250μg/ml, in TE buffer (Tris-HCl, 1mM EDTA, pH 8.0). The bacteriophage λ DNA is fragmented in a restriction digestion with Hind III endonuclease. The digestion reaction results in eight double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g., [32P]-dTTP or [32P]-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase. The product is also available as DNA Molecular Weight Marker II, Digoxigenin-labeled.