Merck ATP Bioluminescence Assay Kit HS II

ATP Bioluminescence Assay Kit HS II

sufficient for 500 assays (tubes), sufficient for 1,000 assays (microplate), kit of 1 (4 components), suitable for detection

Bioluminescence assay

assay

>98%

Quality Level

100

사용

sufficient for 1,000 assays (microplate)
sufficient for 500 assays (tubes)

포장

kit of 1 (4 components)

manufacturer/tradename

Roche

application(s)

detection

배송 상태

dry ice

저장 온도

−20°C

Living things require a continual input of free energy for three major purposes: the performance of mechanical work in muscle contraction and other cellular movements, the active transport of molecules and ions, and the synthesis of macromolecules and other biomolecules from simple precursors.
The free energy used in these processes is derived from the surroundings and maintains an organism in a state that is far from equilibrium. In most processes, this special carrier of free energy is adenosine triphosphate (ATP), an energy-rich molecule. Its triphosphate unit contains two phosphoanhydride bonds. The turnover of ATP is very high. Motion, active transport, signal amplification, and biosynthesis (as is needed for cell proliferation) can occur only if ATP is continuously regenerated from ADP. Therefore, measurement of ATP can serve as a marker for cell proliferation.
The determination of ATP using bioluminescence is a well established technique. It uses the ATP dependency of the light-emitting, luciferase-catalyzed oxidation of luciferin for the measurement of extremely low concentrations of ATP.