Merck First Strand cDNA Synthesis Kit for RT-PCR (AMV)

First Strand cDNA Synthesis Kit for RT-PCR (AMV)

sufficient for 30 reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included

RT-PCR, cDNA sysnthesis

Quality Level

100

사용

sufficient for 30 reactions (including 5 control reactions)

specific activity

>50 units/μg protein

특징

dNTPs included
hotstart: no

포장

kit of 1 (10 components)

manufacturer/tradename

Roche

파라미터

42 °C optimum reaction temp.

technique(s)

RT-PCR: suitable

input

purified RNA

배송 상태

dry ice

저장 온도

−20°C

The use of AMV RT for first-strand cDNA synthesis has certain advantages. The higher thermostability of this enzyme compared to M-MuLV RT allows the reaction to be performed at +42°C, giving higher specificity and better resolution of secondary structures compared to a reaction performed at +37°C.