Merck JumpStart REDTaq ReadyMix Reaction Mix

JumpStart™ REDTaq® ReadyMix™ Reaction Mix

for PCR

Quality Level

200

form

liquid

사용

sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 800 reactions

특징

dNTPs included
hotstart

농도

2.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

색상

red

input

purified DNA

application(s)

agriculture

배송 상태

wet ice

저장 온도

−20°C

JumpStart™ REDTaq® ReadyMix™PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.