Merck Viral RNA Extraction Buffer

Viral RNA Extraction Buffer

One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva

Viral RNA Extraction, Viral Lysis Solution for RT-qPCR, For use with Reverse Transcription-qPCR

Quality Level

200

Grade

Molecular Biology

form

liquid

농도

4X

technique(s)

LAMP: suitable
PCR: suitable
RT-PCR: suitable
qPCR: suitable

배송 상태

wet ice

저장 온도

−20°C

The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.