Merck mRNA Isolation Kit

mRNA Isolation Kit

Quality Level

100

manufacturer/tradename

Roche

포장

kit of for isolation of at least 70 μg of poly (A)+ RNA

The mRNA Isolation Kit isolates highly purified mRNA from cells or tissue.

Affinity purification is a versatile and highly specific technique for the purification of all classes of biomolecules utilizing differences in biological activities of chemical structures. The high selectivity of this technique results in good purification and high recovery. Often a concentrating effect is reached which enables large volumes to be conveniently processed. The mRNA Isolation Kit depends upon the affinity of the poly(A) + tail of mRNA for a biotin-labeled oligo (dT) probe. The probe can pull the mRNA selectively from a lysate without interacting with other RNA or DNA. Once formed, the biotinylated dT-A hybrids can be immobilized on solid surfaces that have been coated with streptavidin, and then washed free of unbound contaminants.