Merck dCas9-3XFLAG-Biotin Protein

dCas9-3XFLAG™-Biotin Protein

from Streptococcus pyogenes with D10A and H840A mutations, recombinant, expressed in E. coli, 1X NLS

Dead Cas9, Cas9 D10A H840A, dCas9, catalytically inactive Cas9

Quality Level

200

재조합

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

lyophilized powder

포장

pkg of 1 kit (3 components)

배송 상태

ambient

저장 온도

−20°C

Recombinant dCas9-3X FLAG-Biotin protein from Streptococcus pyogenes (~165 KD) is a ready-to-use reagent for genome engineering and genomic DNA detection, isolation and imaging experiments. When combined with target-specific guide RNAs, dead Streptococcus pyogenes Cas9 protein acts as a DNA targeting protein suitable for transfection of cell cultures, microinjection, DNA pull-down, chromatin enrichment, microbial identification and in situ DNA imaging. At the C terminus, the protein is tagged with a SV40 large T antigen nuclear localization signal (NLS) for nuclear import and both a 3XFLAG epitope and biotin for anti-FLAG®-based or streptavidin-based experiments.