Merck Cas9-RFP Lenti Plasmid

Cas9-RFP Lenti Plasmid

재조합

expressed in E. coli

form

liquid

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA)

application(s)

CRISPR

리포터 유전자

RFP

selection

ampicillin

배송 상태

dry ice

저장 온도

−20°C

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and RFP linked by a 2A peptide (EF1a-Cas9-2A-RFP) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-RFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed when packaged into lentiviral particles.

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