Merck eSpCas9-GFP Protein

eSpCas9-GFP Protein

from Streptococcus pyogenes with mutations conferring enhanced specificity, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS

eSpCas9-EGFP, eSpCas9-GFP

재조합

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

lyophilized powder

포장

pkg of 1 kit (3 components)

리포터 유전자

GFP

배송 상태

wet ice

저장 온도

−20°C

Recombinant enhanced specificity Cas9-GFP protein from Streptococcus pyogenes (~192 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9-GFP protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

An N-terminally fused enhanced green fluorescent protein with an excitation peak at 488 nm and emission peak at 509 nm allows for visualization of transfected RNP complex in addition to utility in flow cytometry applications. The protein also contains three varied nuclear localization sequences postioned for optimal activity.