Merck Gecko2 Mouse Whole Genome CRISPR Pool, All-in-One Lenti Particles (Gecko2 vector)

Gecko2 Mouse Whole Genome CRISPR Pool, All-in-One™ Lenti Particles (Gecko2 vector)

Quality Level

200

포장

pkg of 8x25 μL (vials)

농도

5x108 VP/ml (via p24 assay)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−70°C

Developed by Feng Zhang′s lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2 vector produces nearly 10X higher titer than version 1. Sigma′s lentiviral mouse GeCKO pool all-in-one is provided in 8 x 25 ul aliquots at a minimum titer of 5X10^8 TU/ml (measured by a p24 assay).

Each species-specific library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together, which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA).