Merck CRISPR Activator Human Oct4 Positive Control Plasmid

CRISPR Activator Human Oct4 Positive Control Plasmid

gRNA Only Expression Plasmid

Quality Level

200

form

liquid

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−20°C

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.