Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

제품 개요

• 타깃 miRNA: mmu-miR-669p-3p
• 디자인: Tough Decoy (TuD) 기반
• 제품 라인: MISSION®
• 형태: Liquid
• 농도: ≥1×10⁶ VP/ml (via p24 assay)
• 배송 상태: Dry ice
• 저장 온도: −70°C

서열 정보

품질 등급

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo). This algorithm employs the Tough Decoy (TuD) design to achieve robust inhibition of target miRNA.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles capable of transducing mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and carries a puromycin resistance gene for stable cell selection.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 다양한 세포 유형에 효율적인 렌티바이러스 전달 가능
• 반복 형질감염 없이 장기적인 억제 유지
• 퓨로마이신 내성 유전자를 통한 안정적 세포 선택 가능