Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-128-1-5p

Tough Decoy (TuD) 디자인 적용

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles suitable for transduction into mammalian cells.

The vector includes:

• Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression
• Puromycin resistance gene for selection of transduced cells

주요 특징

• 강력한 miRNA 억제 효과 제공
• Lentiviral 전달 형식으로 다양한 세포에 효율적 전달 가능
• 반복적인 트랜스펙션 없이 장기간 억제 유지 가능