Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 miRNA

• mmu-miR-3060-5p

디자인

• Tough Decoy (TuD) 기반

제품 라인

• MISSION®

품질 등급

• 200 (M-Clarity Program)

제품 형태 및 사양

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to effectively inhibit target miRNA activity.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which enables co-transfection with compatible packaging plasmids to produce viral particles suitable for mammalian cell transduction.

The vector includes:

• Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression
• Puromycin resistance gene for efficient selection of transduced cells

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 포맷으로 다양한 세포 유형에 적용 가능
• 반복적인 트랜스펙션 없이 장기적 억제 유지 가능