Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-664-5p

Tough Decoy (TuD) Design

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of target miRNA.
miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles for transduction of mammalian cells.
The vector includes the WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 강력한 miRNA 억제 효과
• 다양한 세포 유형에 효율적인 렌티바이러스 전달
• 반복적인 트랜스펙션 없이 장기적 억제 가능

제품 사양

참고 문헌

Haraguchi, T. et al., and Dr. Hideo Iba, University of Tokyo — Tough Decoy (TuD) design algorithm.