Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-2183

Tough Decoy (TuD) Design

제품 사양

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm employs the Tough Decoy (TuD) design to achieve potent inhibition of target miRNAs.

MicroRNAs regulate gene expression through various mechanisms including translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
When co-transfected with compatible packaging plasmids, this vector produces viral particles capable of transducing mammalian cells.

The construct includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) to enhance transgene expression and a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 기반 전달로 다양한 세포 유형에 효율적으로 적용 가능
• 반복 형질감염 없이 장기적 억제 유지 가능
• 퓨로마이신 내성 유전자를 포함하여 안정적인 세포 선택 가능