Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1839-3p

Type: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent and specific inhibition of target miRNA.

miRNA regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
These lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles capable of transducing mammalian cells.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) enhances transgene expression delivered by lentiviral vectors.
The vector also includes a puromycin resistance gene for stable selection of transduced cells.

주요 특징

• 강력하고 선택적인 miRNA 억제 가능
• 렌티바이러스 전달 포맷으로 다양한 세포 유형에 효율적 적용
• 반복적인 트랜스펙션 없이 장기적인 억제 효과 유지
• WPRE 포함으로 발현 효율 향상
• 푸로마이신 저항성 유전자로 안정적 세포 선택 가능