Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1948-3p

Tough Decoy (TuD) Design

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the tough decoy (TuD) design. miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles for efficient transduction of mammalian cells.
The Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) enhances transgene expression, and the lentiviral vector carries a puromycin resistance gene for cell selection.

특징

• Potent inhibition of the desired miRNA
• Efficient delivery into various cell types via lentiviral format
• Enables long-term inhibition without repeat transfection

제품 사양

제품 이미지

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