Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1947-5p

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to effectively inhibit specific miRNA activity.
miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction of mammalian cells.
WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element2) enhances transgene expression.
The vector also includes a puromycin resistance gene for cell selection.

주요 특징

• Potent inhibition of target miRNA
• Efficient delivery via lentiviral system
• Long-term inhibition without repeat transfection
• Puromycin resistance for selection
• Enhanced expression through WPRE inclusion

제품 사양