Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

Target miRNA

mmu-miR-497-5p

Design Type

Tough Decoy (TuD)

제품 라인

MISSION®

품질 등급

200 (M-Clarity Program)

제품 형태

liquid

농도

≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

CAGCAGCACACUGUGGUUUGUA

Sanger 성숙/소수 수납 번호

MIMAT0003453

microRNA 수납 번호

MI0004636

배송 상태

dry ice

저장 온도

−70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of target miRNA.

miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 강력한 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포에 효율적 전달
• 반복적인 트랜스펙션 없이 장기적 억제 유지