Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-714

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design for potent and specific inhibition of target miRNA.

miRNA regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
These lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, co-transfected with compatible packaging plasmids to produce viral particles suitable for mammalian cell transduction.

The vector includes:

• WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element2) for enhanced transgene expression
• Puromycin resistance gene for cell selection

Key Features:

• Potent inhibition of specific miRNA
• Efficient lentiviral delivery across various cell types
• Long-term inhibition without repeat transfection

제품 사양

참고 문헌

Haraguchi, T. et al., University of Tokyo — Development of Tough Decoy (TuD) design for microRNA inhibition.